ABSTRACT
Background: Rostelluria procumbens is a medicinal plant used traditionally in the treatment of asthma, cough and constipation and as an antioxidant etc. it is rich in phytochemical compounds, which are responsible for its biological properties. The present study focused on evaluation of hepatoprotective activity of methanolic extract of R. procumbens leaf in carbon tetrachloride (CCl4) induced hepatotoxocity in rats. Methods: In this study, 30 wistar rats were used and grouped into 6 each group contain 6 rats. In this study, CCl4 is used as hepatotoxin. Four groups were treated with CCl4 and taken as disease control, standard, and two test groups. One group was taken as control treated with saline. Blood samples were collected and estimated serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase and total bilirubin, which are key markers of liver function. The rats were sacrificed and livers were isolated and histopatological studies carried out. Results: On oral administration of methanolic leaves extract of R. procumbens to ethanol intoxicated, rats resulted in significant restoration of enzyme levels and also silymarin at a dose of 25 mg/kg. The reversal of increased serum enzymes in ethanol-induced liver damage by the extract may be due to the prevention of leakage of intracellular enzymes by its membrane stabilizing activity. Conclusion: The results confirm that R. procumbens have hepatoprotective activity against CCl4 induced hepatotoxicity and significant hepatoprotection seen at 500 mg/kg dose.
ABSTRACT
A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of Tinidazole in both pure and pharmaceutical dosage forms. . Chromatographic separation achieved isocratically on a ODS2 reverse phase column [Use Symmetry C18, 250 X 4.6mm, 5μ] utilizing a mobile phase of Methanol. The flow rate was 0.5 ml/min and the effluents were monitored at 310 nm. The retention time was 4.610min. The linearity was in the range of 5-25μg / ml. This method was validated for linearity, precision and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
ABSTRACT
Molecular property is a complex balance of various structural features which determine whether a particular molecule is similar to the known drugs.These properties mainly hydrophobicity, molecular size,flexibility and presence of various pharmacophoric features influence the behavior of molecules in a living organism, including oral bioavailability. This investigation deals with the design and calculation of molecular properties, drug likeness, lipophilicity and solubility parameters of 5-Benzimidazole-1-yl-methyl-[1, 3, 4] oxadiazole-2-thiol and their derivatives using Osiris, mol inspiration ,Mol soft software’s, and ALOPGPS 2.1 program. The compounds followed the Lipinski ‘Rule of five’ for better bioavailability, were synthesized and characterized by IR, NMR, and mass spectral analysis. Furthermore, the binding conformations of these compounds for anti inflammatory activities were determined in silico docking. This is an energy optimization process concerned with the search of the lowest free energy binding mode of a ligand within a protein binding site and estimates the forces involved in the protein-ligand recognition, carried out in Mastro V 2011 in the active site of the cyclooxygenase-2 (COX-2) enzyme.
ABSTRACT
Cefuroxime Axetil is the 1-acetoxyethyl ester of Cefuroxime. Chemically cefuroxime axetil is (RS)-1-hydroxyethyl (6R, 7R)-7-[2-(2-furyl) glyoxyl-amido]- 3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo-Oct-2-ene-2-carboxylate, 72-(Z)-(O-me-thyl-oxime), 1-acetate-3-carbamate. Its molecular formula is C20H22N4O10S, and it has a molecular weight of 510.48. The cefuroxime raw material was identified by HPLC and IR Spectroscopy. The sample is tested for the solubility, diastereoisomer ratio, crystallinity and bulk density. The amount of cefuroxime axetil was estimated by HPLC assay method. The Cefuroxime axetil finished product was identified by HPLC and IR Spectroscopy. The average weight of tablets was calculated by taking the weights of 20 tablets. The content uniformity of the dosage units was calculated by weight variation method. The dissolution rate was calculated by HPLC method by using Paddle apparatus. The amount of Cefuroxime present in the sample was estimated by HPLC assay method. The known and unknown related substances present in the compound were estimated by HPLC method. Results obtained during the study were satisfactory and can be used for commercial purpose.
ABSTRACT
The main aim of the present study was to evaluate analgesic and CNS depressant activity of methanolic extracts of stem and roots of Cissus pallida and aerial parts of Cissus vitegenia in experimental animals. The analgesic activity was evaluated by Eddy’s hot plate method and CNS depressant activity was evaluated by using digital actophotometer. The study was carried out by using two different doses (200 and 400mg/kg body weight) of both the extracts. The preliminary pharmacological screening showed that both the extracts showed moderate analgesic activity and significant CNS depressant activity.